Description 
Non-small cell lung cancer (NSCLC) is a heterogeneous group of cancers encompassing any type of epithelial lung cancer, other than small cell lung cancer (SCLC), which arise from the epithelial cells of the lung and include squamous cell carcinoma, large cell carcinoma, and adenocarcinoma (Thomas, 2023). Recently, oncogenesis in NSCLC has been associated with mutations in the epidermal growth factor receptor (EGFR), or rearrangements of the anaplastic lymphoma kinase (ALK) gene or ROS1 gene (Sequist & Neal, 2023).

For guidance concerning the use of circulating tumor cells in NSCLC, please refer to CAM 235-Liquid Biopsy. 

For guidance concerning Tumor Mutational Burden Testing (TMB) and/or Microsatellite instability (MSI) analysis, please refer to AHS-M2178-Microsatellite Instability and Tumor Mutational Burden Testing policy.

Regulatory Status
The FDA has approved at least 11 tests for assessment of markers relevant to targeted therapy of NSCLC (FDA, 2020a). Additionally, many labs have developed specific tests that they must validate and perform in house. These laboratory-developed tests (LDTs) are regulated by the Centers for Medicare & Medicaid Services (CMS) as high-complexity tests under the Clinical Laboratory Improvement Amendments of 1988 (CLIA ’88). As an LDT, the U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use.

Policy
Application of coverage criteria is dependent upon an individual’s benefit coverage at the time of the request. 

1. For individuals with non-small cell lung cancer (NSCLC), molecular profiling to identify established actionable driver mutations (ALK, BRAF, EGFR, ERBB2(HER2), KRAS, METex14 skipping, NTRK 1/2/3, RET, ROS1) is considered MEDICALLY NECESSARY.
2. To direct therapy in patients with NSCLC, analysis of PD-L1 expression by immunohistochemistry is considered MEDICALLY NECESSARY.
3. As a routine stand-alone assay and as a sole determinant of targeted therapy, KRAS molecular testing is considered NOT MEDICALLY NECESSARY.

The following does not meet coverage criteria due to a lack of available published scientific literature confirming that the test(s) is/are required and beneficial for the diagnosis and treatment of an individual’s illness.

4. To direct targeted therapy for individuals with NSCLC, analysis for genetic alterations in genes not mentioned above is considered NOT MEDICALLY NECESSARY.

NOTES:

Note: For 5 or more gene tests being run on the same platform, please refer to AHS-R2162 Reimbursement Policy.

Table of Terminology

Rationale
 Primary lung cancer is one of the most common malignancies. In the United States, approximately 236,740 individuals are diagnosed and more than 130,180 deaths occur annually. Approximately 95% of lung cancers are either non-small cell or small cell, and 80% – 85% are non-small cell lung cancers (NSCLC) (ACS, 2023; Midthun, 2022). 

Specific molecular treatments for patients based on certain genetic mutations have been developed. Currently, EGFR-, ALK-, ROS1-, BRAF-, and NTRK-positive cases of NSCLC have FDA-approved targeted therapies (i.e., specific treatments for specific mutations), whereas HER2-, MET-, and RET-positive cases are treated with off-label therapies. Therapies for other mutations such as RAS, PTEN, AKT1, and PIK3CA mutations are currently in development. Still, other genetic biomarkers, such as PD-L1 expression and microsatellite instability (MSI) testing may contribute to the management of NSCLC cases (Sequist & Neal, 2023).

EGFR tyrosine kinase mutations are observed in approximately 15% of NSCLC adenocarcinoma cases in the United States and occur more frequently in nonsmokers. The presence of an EGFR mutation usually confers a better prognosis and may be treated by EGFR tyrosine kinase inhibitors (TKIs) such as erlotinib (Sequist & Neal, 2023).

ALK tyrosine kinase translocations are present in approximately 4% of NSCLC adenocarcinoma cases in the United States and occur more frequently in nonsmokers and younger patients. In advanced-stage NSCLC, the presence of an ALK translocation may be treated by ALK TKIs such as crizotinib. Other effective TKIs include ceritinib, alectinib, brigatinib, and lorlatinib (Sequist & Neal, 2023). Studies have indicated that treatment with these therapeutic TKIs significantly prolongs progression-free survival.

ROS1 is a receptor tyrosine kinase that acts as a driver oncogene in 1 to 2% of NSCLC cases by a translocation between ROS1 and other genes such as CD74. ROS1 translocations are usually associated with younger patients and individuals who have never smoked tobacco. Since the ALK and ROS tyrosine kinases are significantly homologous, the ROS1 tyrosine kinase is treatable by ALK TKIs such as crizotinib (Sequist & Neal, 2023).

HER2 (ERBB2) is an EGFR family receptor tyrosine kinase. Mutations in HER2 have been detected in approximately 1 to 3% of NSCLC tumors. These mutations are most frequent in exon 20, resulting primarily in adenocarcinomas and these mutations are more prevalent among individuals who have never smoked tobacco (Sequist & Neal, 2023).

BRAF is a downstream signaling mediator of KRAS that activates the mitogen-activated protein kinase (MAPK) pathway. Activating BRAF mutations have been observed in 1 to 3% of NSCLC cases and are usually associated with smokers. BRAF inhibition with oral small-molecule TKIs has been used to treat this version of NSCLC (Sequist & Neal, 2023).

MET is a tyrosine kinase receptor for hepatocyte growth factor (HGF). MET mutations include MET exon 14 skipping, MET gene amplification, and MET and EGFR co-mutations. Tepotinib has shown evidence of promise in treating MET-exon 14 skipping cases. Crizotinib, an ALK/ROS inhibitor, has also been used to treat MET-exon 14 skipping cases of NSCLC. Other MET-specific therapies are under investigation such as glesatinib and savolitinib. For those with MET amplification, capmatinib or crizotinib are suggested lines of treatment, but are not yet approved for this indication by the FDA and continue to be a line of active research (Sequist & Neal, 2023).

The RET gene encodes a cell surface tyrosine kinase receptor that may be translocated in adenocarcinomas. These mutations occur more frequently in younger patients and in individuals who have never smoked tobacco. Off-label RET inhibitors, such as alectinib, have been used to treat RET-positive cases of NSCLC. In addition, the FDA has approved selpercatinib and pralsetinib for advanced NSCLC in adult patients (Sequist & Neal, 2023).

RAS mutations, in either KRAS or NRAS, are associated with NSCLC. Activating KRAS mutations have been observed in approximately 20 to 25% of lung adenocarcinomas in the United States and are generally associated with smoking. The presence of a KRAS mutation has a limited effect on overall survival in patients with early-stage NSCLC. NRAS is homologous to KRAS and associated with smoking as well; however, NRAS mutations comprise only 1% of NSCLC cases. The clinical significance of NRAS mutations is unclear, and no effective targeted therapies exist at this time (Sequist & Neal, 2023).

PIK3CA, AKT1, and PTEN are three genes involved in the same pathway. PIK3CA encodes the catalytic subunit of phosphatidyl 3-kinase (PI3K), AKT1 acts immediately downstream of PI3K, and phosphatase and tensin homolog (PTEN) inhibits AKT by dephosphorylation. Oncogenic alterations in this pathway include gain-of-function mutations in PIK3CA and AKT1, and loss of PTEN function. PIK3CA mutations may also cause resistance to EGFR TKIs in EGFR-mutated NSCLC. Small-molecule inhibitors of PI3K and AKT are being developed, but clinical efficacy of these agents against specific molecular alterations is unknown (Sequist & Neal, 2023).

Other genetic biomarkers include PD-L1 assessment and microsatellite instability (MSI) testing. Programmed death-1 ligand (PD-L1) expression testing via immunohistochemistry (IHC) is used to guide therapy for patients with NSCLC. Tumor cells present PD-L1 to T-cells to inhibit the immune response by downregulating cytokine production and T-cell proliferation, thereby allowing these tumor cells to evade immune system activity. Monoclonal antibody therapy (immune therapy) has been developed to inhibit this pathway and overcome this mechanism of immune system evasion (Teixidó et al., 2018). Tumor PD-L1 protein expression through immunohistochemistry can be ordered to pinpoint first-line treatment options for NSCLC outside of chemotherapy (Sequist & Neal, 2023).

Microsatellites are short tandem repeat sequences located throughout the genome. However, these sequences are subject to instability caused by faulty mismatch repair genes. This has historically been reported in other cancers, such as Lynch syndrome, and has been reported in NSCLC. MSI testing may be used to evaluate NSCLC cases (Fong et al., 1995).

Precision oncology is now the evidence-based standard of care for the management of many advanced NSCLCs. Expert consensus guidelines have defined minimum requirements for routine testing and identification of EGFR and ALK mutations in advanced lung adenocarcinomas. Targeted use of TKIs based on certain genetic mutations has consistently led to more favorable outcomes compared with traditional cytotoxic agents (Shea et al., 2016). The concept of targeted testing has been approved by the FDA, as package inserts for drugs such as erlotinib specify used for EGFR mutations and other drugs such as pembrolizumab have gained approval for specific types of tumors (in this case, high-MSI tumors) (Boyiadzis et al., 2018; FDA, 2004; Lemery et al., 2017). Proprietary tests are available for identification of relevant mutations, including larger genetic panels. FoundationOne’s 324-gene panel and Oncomine’s 23-gene panel are both FDA-approved as companion diagnostics for non-small cell lung cancer targeted therapies (FDA, 2023). Recently, Guardant 360 CDx was FDA-approved for use as a companion diagnostic to identify NSCLC patients with EGFR mutations who may benefit from Tagrisso (osimertinib) (Guardant, 2021). The company Cobas has a diagnostic approved to identify patients with metastatic NSCLC who might benefit from Tarceva^® (erlotinib) based on formalin-fixed tissue preparation to identify EGFR mutation; a Cobas assay was also FDA approved as a companion diagnostic using liquid biopsy and circulating-free tumor DNA (FDA, 2016).

Clinical Utility and Validity
Lin et al. (2017) evaluated the association between EGFR and EGFR-TKI efficacy in stage IV NSCLC patients. In this study, 94 patients were assessed. The authors calculated a 74.5% objective response rate and a 97.9% disease control rate for EGFR-TKI treatment. The authors concluded that EGFR-TKI therapy resulted in survival benefits for EGFR-mutant patients regardless of “gender, smoking history, pathologic type, type of EGFR mutations, brain metastasis and timing of targeted therapy” (Lin et al., 2017).

Li et al. (2017) examined the effect of number of EGFR mutations on the efficacy of EGFR TKIs. In this study, 201 patients with EGFR mutations were evaluated. These patients were quantitatively separated into “low” and “high” groups based on “amplification refractory mutation system (ARMS) method optimized with competitive blockers and specific mutation quantitation (ARMS+).” The cutoff value was determined by a receiving operating characteristic analysis in a training group and further validated in another group. The investigators found the median progression-free survival (PFS) to be 15 months in the high group compared to two months in the low group. Similar results were reported in the validation group. The authors concluded that the abundance of EGFR mutations was significantly associated with objective response to EGFR TKIs. However, they also noted the abundance of EGFR T790M mutation may adversely affect PFS rather than objective response rate (Li et al., 2017).

Wang et al. (2017) investigated the effect of ALK rearrangements on NSCLC patients. The authors reviewed 15 studies including 4981 patients. The study found that ALK positive (ALK+) patients faced better prognoses (hazard ratio 0.81 of ALK negative patients) except in the non-smoking population (hazard ratio 1.65). ALK+ patients also experienced a significantly higher objective response rate in pemetrexed-based chemotherapy, but not with EGFR-TKI treatment (Wang et al., 2017).

Gainor et al. (2016) performed a study evaluating the efficacy of PD-L1 blockades on EGFR and ALK positive patients. The study evaluated 58 patients; 28 had an EGFR or ALK mutation whereas 30 were wild-type. The investigators found only one of the 28 patients (3.6%) with either mutation had an objective response whereas seven of the 30 (23.3%) wild-type patients had an objective response (Gainor et al., 2016).

Planchard et al. (2016) evaluated the efficacy of the FDA-approved combination of daBRAFenib plus trametinib on previously treated BRAF-mutant metastatic NSCLC. The study included 57 patients; 36 of these patients achieved an overall response. However, serious adverse events were reported in 32 of these patients. The authors concluded that this combination may represent a robust therapy with a management safety profile in BRAF-positive NSCLC patients (Planchard et al., 2016).

A 2019 comprehensive study by Singal et al. (2019) examined the electronic health records (EHR) of 4064 individuals with NSCLC from 275 different oncology practices to explore “associations between tumor genomics and patient characteristics with clinical outcomes …” They note that 21.4% of these individuals had a mutation in EGFR, ALK, or ROS1, and that patients with driver mutations who had targeted therapies had significantly improved overall survival times than individuals who did not have targeted therapies (median of 18.6 versus 11.4 months, respectively); moreover, a tumor mutational burden (TMB) of 20 or higher was associated with improved overall survival for patients on PD-L1-targeted therapy than those patients with a TMB less than 20. The authors concluded that they replicated similar associations from previous research “between clinical and genomic characteristics, between driver mutations and response to targeted therapy, and between TMB and response to immunotherapy” (Singal et al., 2019).

Siena et al. (2019) reported integrated data from three clinical trials focusing on entrectinib. Patients had either ROS1-driven or NTRK-driven cases of NSCLC. Out of 53 patients with ROS1 mutations, approximately 80% responded to entrectinib. Out of 54 patients with NTRK mutations, approximately 60% responded. The authors considered entrectinib to be “tolerable with a manageable safety profile”, and concluded that “entrectinib induced clinically meaningful durable responses in [patients] with ROS1+ NSCLC or NTRK+ solid tumors with or without CNS disease” (Siena et al., 2019).

Volckmar et al. (2019) assessed the “feasibility and clinical utility of comprehensive, NGS-based genetic profiling for routine workup of advanced NSCLC.” The authors based their study on the first 3000 patients seen in their facility. Of the patients tested, the authors identified 807 patients eligible for “currently approved, EGFR-/BRAF-/ALK- and ROS1-directed therapies,” while 218 additional cases with MET, ERBB2 (HER2) and RET alterations could “potentially benefit from experimental targeted compounds.” Other co-mutations such as TP53 and STK11 were also frequently identified, which may be potentially useful predictive and prognostic tools. The authors also noted logistical successes, such as reliability, low dropout rate, fast turnaround times, and minimal tissue requirements. Overall, the authors concluded that this diagnostic approach demonstrated “practicability in order to support individualized decisions in routine patient care, enrollment in molecularly stratified clinical trials, as well as translational research” (Volckmar et al., 2019).

Signorovitch et al. (2019) aimed to evaluate the “budget impact of increased use of CGP [comprehensive genomic profiling] using a 324-gene panel (FoundationOne) vs non-CGP (represented by a mix of conventional molecular diagnostic testing and smaller NGS hotspot panels) and the number needed to test with CGP to gain 1 life year.” The authors developed a decision analytic model to assess the financial impact of increased CGP in advanced non-small cell lung cancer (NSCLC). The study included two million covered lives, of which 532 had advanced NSCLC. Of these patients, 266 underwent molecular diagnostic testing. An increased in CGP among those tested (from 2% – 10%) was associated with a $0.02 per member per month budget impact, of which $0.013 “was attributable to costs of prolonged drug treatment and survival and $0.005 to testing cost.” Overall, the addition of one life year was met with 12 patients tested. The authors concluded that a 2% – 10% increase in CGP use was associated with a “modest budget impact, most of which was attributed to increased use of more effective treatment and prolonged survival” (Signorovitch et al., 2019).

In a prospective study, Peled et al. (2020) investigated the clinical utility of early cell-free deoxyribose nucleic acid (cfDNA) analysis using Guardant 360 CDx in treatment-naive NSCLC patients. Ten patients were studied and the median time from blood draw to receiving the cfDNA results was nine days. Actionable biomarkers were identified in four of the ten patients by both biopsy analysis and cfDNA analysis. Overall, three patients received biomarker-based treatment (one osimertinib, one alectinib, and one crizotinib). The authors concluded that "cfDNA analysis should be ordered by the pulmonologists early in the evaluation of patients with NSCLC, which might complement the tumor biopsy” (Peled et al., 2020).

Al-Ahmadi et al. (2021) studied the overall impact and racial differences of NGS testing in NSCLC patients. The study tested 295 patients with Stage IV NSCLC using the FoundationOne assay and genomic differences were compared by race. "Patients undergoing NGS testing had significantly longer survival of 25.3 months versus those who did not undergo sequencing with a median survival of 14.6 months (P = .002) irrespective if they received targeted therapy or not.” In addition, there was no difference in NGS results based on race. However, African American patients had a higher rate of mutations in PBRM1, SETD2, TSC2, and FBXW7. Overall, there is no racial difference in NGS utilization and testing does increase survival (Al-Ahmadi et al., 2021).

Boeckx et al. (2020) convened a small study of 46 never-smoking, non-small cell lung cancer (NSCLC) patients to investigate genomic alterations in non-smoking individuals. There are few genomic studies focused primarily on this subgroup of patients with NSCLC who have never smoked. Whole exome and low-coverage whole genome sequencing was performed on tumors and matched germline DNA. Fewer somatic mutations, genomic breakpoints, and a smaller percentage of the genome with chromosomal instability in lung tumors were observed in non-smokers compared to smokers. In addition, TSC22D1 was noted as a potential driver gene of NSCLC. The frequency of mutation of TP53, which is associated with negative long-term outcomes, was lower in those who were never-smokers than in smokers. That said, they found driver genes such as EGFR and ERBB2, as well as amplifications in MET were higher in never-smokers. The authors concluded there was a “more favorable prognosis for never smokers with NSCLC” (Boeckx et al., 2020).

National Comprehensive Cancer Network (NCCN)
In the version 3.2023 update for NSCLC, NCCN states that “Numerous gene alterations have been identified that impact therapy selection. Testing of lung cancer specimens for these alterations is important for identification of potentially efficacious targeted therapies, as well as avoidance of therapies unlikely to provide clinical benefit” (NCCN, 2023). NCCN then expounds on their stance, providing a set of “several methodologies are generally considerations for use” that is delineated below.

• “Next-generation sequencing (NGS) is used in clinical laboratories. Not all types of alterations are detected by individual NGS assays and it is important to be familiar with the types of alterations identifiable in individual assays or combination(s) of assays.”
• “It is recommended at this time that when feasible, testing be performed via a broad, panel-based approach, most typically performed by next generation sequencing (NGS). For patients who, in broad panel testing don’t have identifiable driver oncogenes (especially in never smokers), consider RNA-based NGS if not already performed, to maximize detection of fusion event.”
• “Real-time polymerase chain reaction (PCR) can be used in a highly targeted fashion (specific mutations targeted). When this technology is deployed, only those specific alterations that are targeted by the assay are assessed.”
• “Sanger sequencing requires the greatest degree of tumor enrichment. Unmodified Sanger sequencing is not appropriate for detection of mutations in tumor samples with less than 25% to 30% tumor after enrichment and is not appropriate for assays in which identification of subclonal events (eg, resistance mutations) is important. If Sanger sequencing is utilized, tumor enrichment methodologies are nearly always recommended.”
• “Any method that interrogates sequences other than a subset of highly specific alterations (e.g., NGS, Sanger) has the potential to identify variants of uncertain significance (VUS). Any variant classified as a VUS, even if in a gene in which other variants are clinically actionable, should not be considered as a basis for therapy selection.”
• “Other methodologies may be utilized, including multiplex approaches not listed above (i.e., SNaPshot, MassARRAY).”
• “Fluorescence in situ hybridization (FISH) analysis is utilized for many assays examining copy number, amplification, and structural alterations such as gene rearrangements. FISH may have better sensitivity for gene amplification events in some circumstances” (NCCN, 2023).

In order “To minimize tissue use and potential wastage, the NCCN NSCLC Panel recommends that broad molecular profiling be done as part of biomarker testing using a validated test(s) that assesses a minimum of the following potential genetic variants: ALK rearrangements, BRAF mutations, EGFR mutations, KRAS mutations, METex14 skipping mutations, NTRK 1/2/3 gene fusions, RET rearrangements, and ROS1 rearrangements. Both FDA and laboratory-developed test platforms are available that address the need to evaluate these and other analytes. Broad molecular profiling is also recommended to identify rare driver mutations for which effective therapy may be available, such as high-level MET amplifications and ERBB2 mutations” (NCCN, 2023).

The NCCN also states that, “First-line targeted therapy options are recommended for eligible patients with metastatic NSCLC and positive test results for actionable driver mutations such as, ALK, BRAF p.V600E, EGFR, METex14 skipping, NTRK 1/2/3, RET, and ROS1. Second-line targeted therapy is recommended for patients with metastatic NSCLC and positive test results for EGFR exon 20 insertions or KRAS p. G12C mutations” (NCCN, 2023).

In the 2022 NCCN update, the NCCN clarified that “any variant that is classified as VUS should not be used to select targeted therapy even if the VUS occurs in a gene in which other variants are clinically actionable” (NCCN, 2023).

The NCCN Panel added important information about general standards for biomarker testing in eligible patients with NSCLC. They noted that broad molecular profiling is molecular testing that “identifies all of the classic actionable driver mutations described in the algorithm [eg. ALK, BRAF, EGFR, KRAS, METex14 skipping, NTRK 1/2/3, RET, ROS1] — using either a single assay or a combination of a limited number of assays — and optimally also identifies the emerging actionable molecular biomarkers, including high-level MET amplification and ERBB2 mutations. Tiered testing approaches, based on the low prevalence of co-occurring biomarkers, are acceptable” (NCCN, 2023).

EGFR mutations    
EGFR mutations are most often assessed using RT-PCR, Sanger sequencing, and NGS. EGFR mutation status is important for determining use of tyrosine kinase inhibitor (TKI) therapies. EGFR mutations include, but are not limited to, exon 19 deletions, p.L858R point mutation, p.L861Q, p.G719X, p.S768I0, exon 20 insertion variants, and p.T790M. As a category 1 recommendation, EGFR mutation testing is recommended for advanced or metastatic disease, including adenocarcinoma, large cell, and NSCLC NOS. As a category 2A recommendation, it is recommended to consider it for individuals with squamous cell carcinoma who have never been smokers, small biopsy specimens, or mixed histology (NCCN, 2023).

ALK gene rearrangements
ALK gene rearrangements are most often assessed using FISH, but IHC can also be effective. The NCCN states that NGS can detect ALK fusions, but PCR is less likely to detect any ALF fusion with a novel partner(s). The most common fusion partner for ALK is EML4; however, other partners have been isolated and identified. Like EGFR, ALK status is used in determining whether TKI therapies are appropriate. As a category 1 recommendation, ALK testing is recommended for advanced or metastatic disease, including adenocarcinoma, large cell, and NSCLC NOS. As a category 2A recommendation, it is recommended to consider it for individuals with squamous cell carcinoma who have never been smokers, small biopsy specimens, or mixed histology (NCCN, 2023).

ROS1 rearrangements
In NSCLC, ROS1 rearrangements can result in inappropriate ROS1 kinase signaling. Similar to ALK, FISH break-apart testing is often used, but this methodology “may under-detect the FIG-ROS1 variant” (NCCN, 2023). IHC requires confirmatory testing because it has a low specificity for ROS1. PCR, if used, can be unlikely to detect novel fusion partners. The use of NGS can detect ROS1 fusions, but DNA-based NGS is prone to under-detection of ROS1 fusions. ROS1 status is important for responsiveness to oral ROS1 TKIs. As category 2A recommendations, ROS1 testing should be performed for advanced or metastatic disease, including adenocarcinoma, large cell, and NSCLC NOS; it should be considered in individuals with squamous cell carcinoma with small biopsy specimens or mixed histology. Entrectinib has been noted as a preferred treatment option for ROS1 rearrangements in advanced or metastatic NSCLC by the NCCN since 2019. However, it should be noted that “Targeted real-time PCR assays are utilized in some settings, although they are unlikely to detect fusions with novel partners” (NCCN, 2023).

BRAF point mutations
Sequencing methods, especially NGS and Sanger (ideally paired with tumor enrichment), and rtPCR are most often used for detecting BRAF point mutations. BRAF V600 mutations are associated with responsiveness to certain combination therapies. Many BRAF mutations have been identified in NSCLC, but the impact of these mutations is not well-understood as of date. As category 2A recommendations, BRAF testing should be performed for advanced or metastatic disease, including adenocarcinoma, large cell, and NSCLC NOS; it should be considered in individuals with squamous cell carcinoma with small biopsy specimens or mixed histology (NCCN, 2023).

KRAS point mutations
Like BRAF, sequencing methods are used in the identification of point mutations within the KRAS gene. For NSCLC, the most common KRAS mutations are located in codon 12 even though other point mutations may occur elsewhere. KRAS mutations have been linked as a prognostic indicator of poor survival and can impact EGFR TKI therapy. The NCCN states, “EGFR, KRAS, ROS1, and ALK genetic alterations do not usually overlap; thus, testing for KRAS mutations may identify patients who will not benefit from further molecular testing.” A newly designed oral KRAS G12C inhibitor was found to be effective in use against the KRAS p.G12C mutation, but this class of inhibitor has not been evaluated for any other mutations (NCCN, 2023).

MET exon 14 skipping variants
NGS-based testing, particularly RNA-based as it provides improved detection, is used to detect METex14 skipping events. Immunohistochemistry is not used. Oral TKI therapy is used to address a METex14 skipping mutation when detected. The NCCN states that “NGS-based testing is the primary method for detection of METex14 skipping events; RNA-based NGS may have improved detection. IHC is not a method for detection of METex14 skipping” (NCCN, 2023).

RET
FISH break-apart probe methodology is one appropriate method used to detect a RET mutation, though it may under-detect some fusions. NCCN also states that “Targeted real-time reverse-transcriptase PCR assays are utilized in some settings, although they are unlikely to detect fusions with novel partners. NGS-based methodology has a high specificity, and RNA-based NGS is preferable to DNA-based NGS for fusion detection.” Sequencing methods such as NGS and rtPCR are effective but rtPCR has difficulty detecting fusions with novel partners. RNA-based NGS has better fusion detection capability than DNA-based NGS. Regardless of fusion partner, RET mutations are responsive to oral RET TKI therapies (NCCN, 2023).

PD-L1
PD-L1 is expressed on tumor cells; its presence is used to determine possible pembrolizumab therapy. The FDA has approved IHC use for assessing PD-L1. The FDA-approved companion diagnostic for PD-L1 guides utilization of pembrolizumab in patients with NSCLC and is based on the tumor proportion score. As a category 1 recommendation, PD-L1 testing is recommended for all cases of advanced or metastatic disease, including adenocarcinoma, large cell, NSCLC NOS, and squamous cell carcinoma. NCCN states that, in comparison to TMB, “PD-L1 expression level is a more useful immune biomarker than TMB for deciding how to use immunotherapy, because test results are obtained more quickly, less tissue is needed for testing, and data demonstrate relative reproducibility across platforms and individuals.” However, “While some clones for PD-L1 IHC are FDA-approved for specific indications, use of multiple IHC tests is not necessary, provided any individual IHC test has been internally validated for comparability for categorical results against the FDA-approved clone” (NCCN, 2023).

NTRK gene fusion
The NCCN has an NTRK gene fusion positive algorithm where larotrectinib is to be used as a first-line therapy if the gene fusion was discovered prior to first-line systemic therapy. If the NTRK gene fusion was discovered during a different first-line systemic therapy, then they recommend completing the planned systemic therapy, including maintenance therapy, and then follow this first-line therapy up with larotrectinib. As a category 2A recommendation, the NCCN recommends NTRK gene fusion testing to be included as part of molecular profiling for all forms of advanced or metastatic disease, including adenocarcinoma, large cell, NSCLC NOS, and squamous cell carcinoma. The NCCN NSCLC Panel recommends larotrectinib and entrectinib (both are category 2A) as either first-line or subsequent therapy options for patients with NTRK gene fusion-positive metastatic NSCLC based on data and the FDA approvals. As of the v3 2020 update, both agents are considered “preferred” first-line therapies for patients with NTRK gene fusion-positive metastatic disease (NCCN, 2023).

Tumor Mutational Burden (TMB)
NCCN reports that “In 2020, the NCCN Panel deleted tumor mutational burden (TMB) as an emerging immune biomarker based on clinical trial data and other issues”. Preliminary data from PFS from CHECKMATE 227, a phase 3 randomized trial with a complex design, had suggested that TMB might be a useful immune biomarker for deciding whether to use immunotherapy in patients with metastatic NSCLC, but updated data indicated that “overall survival was improved with nivolumab plus ipilimumab regardless of TMB or PD-L1 expression levels”. Furthermore, “Several trials have shown that high TMB levels do not correlate with PD-L1 expression levels in patients with NSCLC." This lack of clinical data, coupled with technical problems with measuring TMB — including “: 1) lack of agreement on the definition of a cut off for designating high TMB levels; and 2) lack of standardization of TMB measurements across laboratories” — drives the NCCN Guidelines to “not recommend measurement of TMB levels before deciding whether to use nivolumab plus ipilimumab regimens or to use other ICIs, such as pembrolizumab” (NCCN, 2023).

Emerging biomarkers to identify novel therapies
The NCCN version 2.2022 also lists the following emerging biomarkers to identify novel therapies for patients with metastatic NSCLC.

College of American Pathologists (CAP), the International Association for the Study of Lung Cancer (IASLC), and the Association for Molecular Pathology (AMP)
The CAP/IASLC/AMP joint guidelines indicate that “EGFR molecular testing should be used to select patients for EGFR-targeted TKI [tyrosine kinase inhibitor] therapy” (Evidence Grade: A) (Lindeman et al, 2013). Testing is recommended for adenocarcinomas and mixed lung cancers “regardless of histologic grade.” However, in the setting of fully excised lung cancer specimens, EGFR testing is not recommended for lung cancer without any adenocarcinoma component (Evidence Grade: A). In the setting of more limited lung cancer specimens where an adenocarcinoma component cannot be completely excluded, EGFR testing is recommended “in cases showing squamous or small cell histology but clinical criteria (e.g., young age, lack of smoking history) may be useful in selecting a subset of these samples for testing” (Evidence Grade: A)” (Lindeman et al, 2013). The 2018 CAP guidelines reaffirmed the 2013 guideline recommendations of universal testing of lung cancer patients with advanced-stage cancers with an adenocarcinoma component, using molecular diagnosis for activating ‘‘hot-spot’’ mutations in EGFR exons 18 to 21 with at least 1% prevalence (i.e., codons 709 and 719, exon 19 deletion 768, and exon 20 insertions 790, 858, and 861) (Lindeman et al., 2018).

CAP also added the recommendation that: “In lung adenocarcinoma patients who harbor sensitizing EGFR mutations and have progressed after treatment with an EGFR-targeted tyrosine kinase inhibitor, physicians must use EGFR T790M mutational testing when selecting patients for third-generation EGFR-targeted therapy. Laboratories testing for EGFR T790M mutation in patients with secondary clinical resistance to EGFR targeted kinase inhibitors should deploy assays capable of detecting EGFR T790M mutations in as little as 5% of viable cells” (Lindeman et al., 2018).

The CAP recommendations were updated to include “3 categories into which genes should be placed. One set of genes must be offered by all laboratories that test lung cancers, as an absolute minimum: EGFR, ALK, and ROS1. A second group of genes should be included in any expanded panel that is offered for lung cancer patients: BRAF, MET, RET, ERBB2 (HER2), and KRAS, if adequate material is available...All other genes are considered investigational at the time of publication.” They elaborate to recommend: “In this context, institutions providing care for lung cancer patients have a choice: (1) offer a comprehensive cancer panel that includes all of the genes in the first 2 categories (EGFR, ALK, ROS1, BRAF, MET, ERBB2 [HER2], KRAS, RET) for all appropriate patients, or (2) offer targeted testing for the genes in the must-test category (EGFR, ALK, ROS1) for all appropriate patients and offer as a second test an expanded panel containing the second-category genes (BRAF, MET, ERBB2 [HER2], and RET) for patients who are suitable candidates for clinical trials, possibly after performing a single-gene KRAS test to exclude patients with KRAS-mutant cancers from expanded panel testing” (Lindeman et al., 2018). However, the CAP states that “KRAS molecular testing is not indicated as a routine stand-alone assay as a sole determinant of targeted therapy. It is appropriate to include KRAS as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative” and that “[RET, MET, KRAS, and ERBB (HER2)] molecular testing is not indicated as a routine stand-alone assay outside the context of a clinical trial. It is appropriate to include [RET, MET, KRAS, and ERBB (HER2)] as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative” (Lindeman et al., 2018).

The guidelines indicate that “ALK molecular testing should be used to select patients for ALK-targeted TKI therapy” (Evidence Grade: B) (Lindeman et al., 2013). Testing is recommended for adenocarcinomas and mixed lung cancers “regardless of histologic grade.” However, in the setting of fully excised lung cancer specimens, ALK testing is not recommended for lung cancer without any adenocarcinoma component (Evidence Grade: A). In the setting of more limited lung cancer specimens where an adenocarcinoma component cannot be completely excluded, ALK testing is recommended “in cases showing squamous or small cell histology but clinical criteria (eg, young age, lack of smoking history) may be useful in selecting a subset of these samples for testing” (Evidence Grade: A) (Lindeman et al., 2013).

The CAP recommends that “Multiplexed genetic sequencing panels are preferred over multiple single-gene tests to identify other treatment options beyond EGFR, ALK, and ROS1.” They found that “NGS enables the simultaneous assessment of all 3 of the ‘‘must-test’’ genes in lung cancer — EGFR, ALK, ROS1 — as well as each of the genes suggested for inclusion in larger panels — BRAF, RET, ERBB2 (HER2), KRAS, MET — and hundreds to thousands of other genes that may have potential roles in cancer development. In addition to small mutations, NGS assays can detect fusions/rearrangements and copy number changes in the targeted genes, if designed with these alterations in mind. Numerous studies have demonstrated the excellent sensitivity of NGS methods relative to single-gene targeted assays, particularly for single-nucleotide-substitution mutations. Next-generation sequencing methods typically require less input DNA and can accommodate smaller samples with lower concentrations of malignant cells, and, although typically slower than 1 single-gene assay, can often be performed more rapidly than sequential multiple single-gene assays. A reduced need for repeat biopsy is an additional benefit of panel testing” (Lindeman et al., 2018).

When asked about new genes that should be tested for in lung cancer patients, CAP strongly recommends that “ROS1 testing must be performed on all lung adenocarcinoma patients, irrespective of clinical characteristics” (Lindeman et al., 2018).
 

Other guidance was backed by expert consensus opinion, and these recommendations include the following:

• “ROS1 IHC may be used as a screening test in lung adenocarcinoma patients; however, positive ROS1 IHC results should be confirmed by a molecular or cytogenetic method.”
• “BRAF molecular testing is currently not indicated as a routine stand-alone assay outside the context of a clinical trial. It is appropriate to include BRAF as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative.”
• “"RET molecular testing is not recommended as a routine stand-alone assay outside the context of a clinical trial. It is appropriate to include RET as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative.”
• “ERBB2 (HER2) molecular testing is not indicated as a routine stand-alone assay outside the context of a clinical trial. It is appropriate to include ERBB2 (HER2) mutation analysis as part of a larger testing panel performed either initially or when routine EGFR, ALK, and ROS1 testing are negative.”
• “KRAS molecular testing is not indicated as a routine stand-alone assay as a sole determinant of targeted therapy. It is appropriate to include KRAS as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative.”
• “MET molecular testing is not indicated as a routine stand-alone assay outside the context of a clinical trial. It is appropriate to include MET as part of larger testing panels performed either initially or when routine EGFR, ALK, and ROS1 testing are negative” (Lindeman et al., 2018).

In 2018, CAP added the recommendation that “IHC is an equivalent alternative to FISH for ALK testing”, and that “although at the time of writing RT-PCR and NGS are not approved by the FDA in the United States as first-line methods for determining ALK status in selection of patients for ALK inhibitor therapy, these approaches have shown comparable performance with IHC when designed to detect the majority of fusions, and are standard practice in many other countries. These methods are highly specific for most fusions, and patients with positive results should be treated with an ALK inhibitor, although patients with negative results may benefit from a more sensitive method to exclude the possibility of a variant fusion. Similarly, amplicon-based NGS assays of DNA may likewise fail to detect all fusion variants, and therefore a capture-based DNA or RNA approach is preferred for NGS testing for ALK fusions. Current data are still too limited to develop a specific recommendation either for or against the use of NGS for ALK fusions as a sole determinant of ALK TKI therapy” (Lindeman et al., 2018).

When performing molecular testing, CAP suggests that “Multiplexed genetic sequencing panels are preferred over multiple single-gene tests to identify other treatment options beyond EGFR, ALK, and ROS1” and that “Laboratories should ensure test results that are unexpected, discordant, equivocal, or otherwise of low confidence are confirmed or resolved using an alternative method or sample” (Lindeman et al., 2018). 

CAP states that testing indicated for patients with targetable mutations who have relapsed on targeted therapy include

• “In lung adenocarcinoma patients who harbor sensitizing EGFR mutations and have progressed after treatment with an EGFR-targeted tyrosine kinase inhibitor, physicians must use EGFR T790M mutational testing when selecting patients for third-generation EGFR-targeted therapy” (strong recommendation).
• “Laboratories testing for EGFR T790M mutation in patients with secondary clinical resistance to EGFR-targeted kinase inhibitors should deploy assays capable of detecting EGFR T790M mutations in as little as 5% of viable cells” (recommendation).

However, CAP also finds that “There is currently insufficient evidence to support a recommendation for or against routine testing for ALK mutational status for lung adenocarcinoma patients with sensitizing ALK mutations who have progressed after treatment with an ALK-targeted tyrosine kinase inhibitor” (Lindeman et al., 2018).

Regarding the use of circulating cell-free DNA, CAP claims that, “There is currently insufficient evidence to support the use of circulating cell-free plasma DNA molecular methods for the diagnosis of primary lung adenocarcinoma.” Moreover, “There is currently insufficient evidence to support the use of circulating tumor cell molecular analysis for the diagnosis of primary lung adenocarcinoma, the identification of EGFR or other mutations, or the identification of EGFR T790M mutations at the time of EGFR TKI resistance.” However, CAP concedes that “In some clinical settings in which tissue is limited and/or insufficient for molecular testing, physicians may use a cell-free plasma DNA assay to identify EGFR mutations” and that “Physicians may use cell-free plasma DNA methods to identify EGFR T790M mutations in lung adenocarcinoma patients with progression or secondary clinical resistance to EGFR-targeted tyrosine kinase inhibitors; testing of the tumor sample is recommended if the plasma result is negative” (Lindeman et al., 2018).
 

American Society of Clinical Oncology (ASCO) 
The ASCO published a joint update on “Therapy for Stage IV Non Small-Cell Lung Cancer Without Driver Alterations” with Ontario Health (OH). These guidelines are intended for patients without alterations in EGFR or ALK. These recommendations divide PD-L1 expression into three categories: negative (0%), low positive (1 – 49%) and high (> 50%). Pembrolizumab, carboplatin, pemetrexed, atezolizumab, paclitaxel, and bevacizumab are all listed as potential treatments, some of which may stand alone and some which are to be used in combination (Hanna et al., 2021).

Another joint update with Cancer Care Ontario remarked that “Mutations in KRAS are not predictive for benefit from adjuvant chemotherapy” (Kris et al., 2017).

ASCO published an endorsement of the joint guidelines from the CAP/IASLC/AMP with minor modifications. Relevant differences from the joint guidelines include:

• BRAF testing should be performed on all patients with advanced lung adenocarcinoma, irrespective of clinical characteristics.
• Physicians may use molecular biomarker testing in tumors with an adenocarcinoma component or nonsquamous nonsmall-cell histology (in addition to “any nonsmall-cell histology when clinical features indicate a higher probability of an oncogenic driver (e.g., young age [50 years]; light or absent tobacco exposure)” (Kalemkerian et al., 2018).

European Society for Medical Oncology (ESMO) 
According to ESMO, genetic alterations, which are key oncogenic events (driver mutations), have been identified in NSCLC, with two of these — EGFR mutations and the anaplastic lymphoma kinase (ALK) rearrangements — determining approved, selective pathway-directed systemic therapy. The ESMO guidelines do not specifically mention KRAS mutation testing. NGS is also mentioned for ALK, RET, ROS1, MET, HER2, and BRAF mutations (Novello et al., 2016).

ESMO remarks that the role of targeted agents in stages I – III NSCLC have not been evaluated properly. Therefore, they state that “there is no role for targeted agents in stage III NSCLC outside clinical trials” (Postmus et al., 2017).

ESMO published a guideline regarding metastatic NSCLC in 2020. In it, they note EGFR, ALK, ROS1, BRAF, and PD-L1 expression as usable biomarkers for “personalised medicine.” HER2, MET, NTRK, and RET are considered “evolving targets/biomarkers.” ESMO’s specific recommendations are listed below:

• “EGFR mutation status should be systematically analysed in advanced NSCC [non-small cell lung cancer] [level of evidence “I”, strength of recommendation “A”]. Test methodology should have adequate coverage of mutations in exons 18 – 21, including those associated with resistance to some therapies [III, B]. At a minimum, when resources or material are limited, the most common activating mutations (exon 19 deletion, exon 21 L858R point mutation) should be determined”
• “The availability of TKIs effective against T790M-mutant recurrent disease makes T790M testing on disease relapse mandatory [I, A]”
• “Testing for ALK rearrangement should be systematically carried out in advanced non-squamous NSCLC [I, A]”
• “Testing for ROS1 rearrangement should be systematically carried out in advanced NSCLC [III, A]. Detection of the ROS1 translocation by FISH remains a standard; IHC may be used as a screening approach [IV, A]”
• “BRAF V600 mutation status should be systematically analysed in advanced NSCLC for the prescription of BRAF/MEK inhibitors”
• “Molecular EGFR and ALK testing are not recommended in patients with a confident diagnosis of SCC, except in unusual cases, e.g., never/former light smokers or long-time ex-smokers”
• “If available, multiplex platforms (NGS) for molecular testing are preferable [III, A].”
• “PD-L1 IHC should be systematically determined in advanced NSCLC [I, A]”
• “Testing is required for pembrolizumab therapy but may also be informative when nivolumab or atezolizumab are used” (Planchard et al., 2020). 

In 2023, ESMO issued clinical practice guidelines focusing on oncogene-addicted metastatic non-small-cell lung cancer. These guidelines include

• “Adequate tissue material for histological diagnosis and molecular testing should be obtained to allow for individual treatment decisions [IV, A].”
• “Pathological diagnosis should be made according to the 2021 World Health Organization classification of lung tumours [IV, A].
• “Specific subtyping of all NSCLCs is necessary for therapeutic decision making and should be carried out wherever possible. IHC stains should be used to reduce the NSCLC-not otherwise specified rate to fewer than 10% of cases diagnosed [IV, A].
• The molecular tests below are recommended in patients with advanced non-squamous-cell carcinoma, and not recommended in patients with a confident diagnosis of squamous-cell carcinoma, except in unusual cases, e.g. young (< 50 years) patients, never (< 100 cigarettes in a lifetime)/former light smokers (≤ 15 pack-years, all kinds of tobacco) or long-time ex-smokers (quit smoking > 15 years ago, all kinds of tobacco) [IV, A].
• EGFR mutation status should be determined [I, A]. Test methodology should have adequate coverage of mutations in exons 18 – 21, including those associated with resistance to some therapies [III, A]. At a minimum, when resources or material are limited, the most common activating mutations (exon 19 deletion, exon 21 L858R point mutation) should be determined [I, A].
• The availability of TKIs effective against T790M-mutated recurrent disease makes T790M testing on disease relapse on first- or second-generation EGFR TKIs mandatory [I, A].
• Testing for ALK rearrangements should be carried out [I, A].
• Detection of the ALK translocation by FISH remains a standard, but IHC with high-performance ALK antibodies and validated assays may be used for screening [III, A] and have been accepted as an equivalent alternative to FISH for ALK testing.
• Testing for ROS1 rearrangements should be carried out [II, A]. Detection of the ROS1 translocation by FISH remains a standard; IHC may be used as a screening approach [IV, A].
• BRAF V600 mutation status testing should be carried out [II, A].
• Testing for NTRK rearrangements should be carried out [II, A]. Screening for NTRK rearrangements may use IHC or NGS, with appropriate testing follow-up to validate a positive result [II, A].
• Testing for MET exon 14 skipping mutations, MET amplifications, RET rearrangements, KRAS G12C mutations and HER2 mutations should be carried out [II, A].
• If available, multiplex platforms (NGS) for molecular testing are preferable [III, A].
• RNA-based NGS is preferred for identifying an expanding range of fusion genes [III, B]. Whichever testing modality is used, it is mandatory that adequate internal validation and quality control measures are in place and that laboratories participate in, and perform adequately in, external quality assurance schemes for each biomarker test [III, A].
• cfDNA (liquid biopsy) can be used to test for oncogenic drivers as well as resistance mutations, but all patients with a negative cfDNA blood test still require tissue biopsy [II, A]” (Hendriks et al., 2023).

National Institute for Health and Care Excellence (NICE)
NICE has provided guidance for EGFR‑TK mutation testing in adults with locally advanced or metastatic non-small-cell lung cancer.

“1.1  The tests and test strategies listed below are recommended as options for detecting epidermal growth factor receptor tyrosine kinase (EGFR‑TK) mutations in the tumours of adults with previously untreated, locally advanced or metastatic non-small-cell lung cancer (NSCLC), when used in accredited laboratories participating in an external quality assurance scheme. The laboratory-developed tests should be designed to detect the mutations that can be detected by one of the CE‑marked tests as a minimum.

• Therascreen EGFR RGQ PCR Kit (CE‑marked, Qiagen)
• Cobas EGFR Mutation Test (CE‑marked, Roche Molecular Systems)
• Sanger sequencing of samples with more than 30% tumour cells and therascreen EGFR RGQ PCR Kit for samples with lower tumour cell contents
• Sanger sequencing of samples with more than 30% tumour cells and cobas EGFR Mutation Test for samples with lower tumour cell contents
• Sanger sequencing followed by fragment length analysis and polymerase chain reaction (PCR) of negative samples.

1.2    There was insufficient evidence for the Committee to make recommendations on the following methods:

• High-resolution melt analysis
• Pyrosequencing combined with fragment length analysis
• Single-strand conformation polymorphism analysis
• Next-generation sequencing
• Therascreen EGFR Pyro Kit (CE‑marked, Qiagen)” (NICE, 2013)

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Coding Section

Procedure and diagnosis codes on Medical Policy documents are included only as a general reference tool for each policy. They may not be all-inclusive. 

This medical policy was developed through consideration of peer-reviewed medical literature generally recognized by the relevant medical community, U.S. FDA approval status, nationally accepted standards of medical practice and accepted standards of medical practice in this community, Blue Cross Blue Shield Association technology assessment program (TEC) and other nonaffiliated technology evaluation centers, reference to federal regulations, other plan medical policies and accredited national guidelines.

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